To test if SE accumulation in Hsl1 mutants is accompanied by misexpression of other sterol metabolic genes, we assessed mRNA concentrations of the sterol-responsive transcription factor Hr96, the Niemann-Pick-related genes Npc1a, Npc1b, Npc2a, and Npc2b, the putative sterol-O-acyltransferase gene CG8112 and the intestinal SE hydrolase gene magro. We observed a 1.8-fold increase in magro mRNA levels in Hsl1 mutants as compared to controls, whereas expression of all other sterol metabolic genes investigated remained largely unaltered in response to Hsl-deficiency (Figure 3H). This evidence concerns the gene DCPS and hyperinsulinemic hypoglycemia, familial, 4.