The results show that the amount of KIF5B in the KIF5B siRNA condition changed significantly compared to that in the control condition at 0 h or 1 h PEDV post-infection, while the PEDV N protein did not, illustrating that KIF5B was successfully knocked down in Vero cells, but its knockdown almost had no obvious influence on the PEDV attachment or internalization. This evidence concerns the gene KIF5B and infection.