In addition, CD133+/CD49f+ tumor stem cells isolated from alcohol-fed HCV Ns5a or core transgenic mice, are tumorigenic based on the roles of TLR4 and Nanog, which is correlated with TGF-β signaling pathway due to Nanog-mediated expression of IGF2BP3 and YAP1 66. This evidence concerns the gene PROM1 and neoplasm.