Moreover, we confirmed the molecular details of connections among hub genes (PGM2, PODXL, RHNO1, SCD, and SEPHS1) and three key pathways (“Cell cycle,” “Purine metabolism,” and “Spliceosome”) and a co-expression relationship between hub genes and TFs to obtain a better understanding of molecular mechanisms involved in CRC. Here, RHNO1 is linked to colorectal carcinoma.