Our research group has recently demonstrated that Acute Restraint Stress (ARS) induces anxiety-like behavior, associated with GABAergic dysfunction in the zebrafish brain (Assad et al., 2020), and based on these findings; the present study evaluated the preventive effects of treatment with the CB1 agonist on the neurochemistry of anxiety-like behavior induced by ARS in zebrafish. Here, CNR1 is linked to Anxiety.