At week 16 Ccl2 [also known as monocyte chemoattractant protein 1 (Mcp1)], Pdgfb and Col1a1 were increased approximately 3.5-, 2- and 2.5-fold in Charles River animals with NASH compared to Envigo, respectively (p = 0.0030, p = 0.0008 and p = 0.0253, respectively) (Fig. 5A). The gene discussed is COL1A1; the disease is metabolic dysfunction-associated steatohepatitis.