At week 24/25, the inflammatory genes Ccl2 and Cxcl8 (interleukin 8) were increased approximately 4- and 3-fold in Charles Rivers NASH animals (p = 0.0002 and p = 0.004) while Tnf was nominally increased compared to Envigo animals with NASH (p = 0.0552) (Fig. 5B). This evidence concerns the gene CCL2 and metabolic dysfunction-associated steatohepatitis.