Immunofluorescence microscopy of human metastatic GC biopsy samples showed profound increase of Siah2, P-Ser6-Siah2 and P-Thr279-Siah2 but decreased MRCKβ in metastatic GC samples as compared to their paired normal gastric tissues (Fig. 5c and Additional file 1: Fig. S3i–iii). This evidence concerns the gene SIAH2 and gastric cancer.