To elucidate the effects of GnRH produced and secreted by the tumor cells, we treated cell cultures of human ovarian cancer cell lines EFO-21 and EFO-27 that had been shown to express high affinity GnRH-R and to secrete GnRH with neutralizing concentrations of antiserum to GnRH. The gene discussed is GNRH1; the disease is ovarian carcinoma.