To examine whether the Ms_Rv0580c regulates the HIF-1α and ER stress marker genes, we pre-treated the THP-1 macrophages with TPCK (NF-κB inhibitor), SP600125 (JNK inhibitor) and SB202190 (p38 inhibitor), separately, for 1 h before infection with Ms_Rv0580c and Ms_pNIT. This evidence concerns the gene NFKB1 and infection.