It has been suggested that NSCLC cell lines possess driver mutations or gene amplification alterations that are not associated with FOLR1, such as the epidermal growth factor receptor (EGFR)-activating mutation found in the PC-9, HCC827, H1650, HCC4006, and H1975 cell lines; the RAS mutation in the PC-14 and A549 lines; and MET amplification in the NCI-H441 and ABC-1 lines [40]. The gene discussed is EGFR; the disease is non-small cell lung carcinoma.