ChIP-qPCR analysis showed that the DNA-binding affinity of STAT3 was significantly reduced at the known target genes such as Snail [10], Spg20 [11], Mmp3 [12] in the colon cancer cells with STAT3del, especially in the SW480 cells with STAT3del (Figure 2C). Here, STAT3 is linked to malignant colon neoplasm.