Therefore, in this study, we deleted a sequence encoding amino acids 400-411 in the DNA-binding domain of STAT3 (STAT3del) using the CRISPR-CAS9 gene editing system in multiple colon cancer cell lines (SW480, SW620, and HCT116) and characterized its effects on genome-wide binding to STAT3-target genes using ChIP-seq analysis. This evidence concerns the gene STAT3 and malignant colon neoplasm.