When we evaluated NT-proBNP cutoffs at 152 pg/ml (5th percentile) and 269 pg/ml (10th percentile) in patients with heart failure and AF (n = 268) in comparison with the recommended cutoff of <125 pg/ml, the recommended cutoff accurately ruled out heart failure in all patients with AF, whereas higher thresholds were less accurate (Table E in S1 Text). The gene discussed is NPPB; the disease is heart failure.