As shown in Fig. 5D, the association of actin with the TJ adaptor protein ZO-1 decreased in a dose-dependent manner in Sertoli cells after ZIKV infection or ZIKV E transfection, prompting that the weakened interaction between actin and ZO-1 might have contributed to the decrease in TEER. The gene discussed is TJP1; the disease is Zika virus infectious disease.