In order to further study the effect of GSK3β on the growth of CRC xenograft tumours in vivo by mediating the expression of c‐Myc, the stably transfected SW620 cell lines were screened, and the stably transfected SW620 cell suspension was injected subcutaneously to establish the subcutaneous xenograft model of nude mice. The gene discussed is GSK3B; the disease is neoplasm.