GPHN and Epileptic encephalopathy: In the present study, we performed atomic-scale molecular dynamics (MD) simulations combined with thermodynamic integration (TI) calculations for wild-type (WT) gephyrin and a subset of gephyrin missense mutants associated with ASDs or epileptic encephalopathy, to test the hypothesis that these mutations affect the structural stability of the multimeric gephyrin complex.