These Smad proteins activated by phosphorylation acts as transcription factors by assembling with Smad4 and regulates cell proliferation, migration, and differentiation.50AHNAK has diverse role as oncogene or tumour-suppressor gene.51,52AHNAK promotes EMT via TGFB/Smad signalling pathway and regulates cell migration and metastasis.53 Additionally, we revealed lower expression of AKNAK and TGFB1 in ETNK2 KO cell lines. This evidence concerns the gene TGFB1 and neoplasm.