These Smad proteins activated by phosphorylation acts as transcription factors by assembling with Smad4 and regulates cell proliferation, migration, and differentiation.50AHNAK has diverse role as oncogene or tumour-suppressor gene.51,52AHNAK promotes EMT via TGFB/Smad signalling pathway and regulates cell migration and metastasis.53 Additionally, we revealed lower expression of AKNAK and TGFB1 in ETNK2 KO cell lines. Here, SMAD4 is linked to neoplasm.