HMGB1 and cancer: IMMUNEPOTENT CRP (ICRP), a bovine dialysable leukocyte extract (DLE) obtained from disrupted spleen, is cytotoxic to several cancer cell lines,8–11 without affecting the viability of non-cancer cells.11 ICRP induces ICD in the murine melanoma model B16F10,12 whereas in HeLa and MCF-7 cells, ICRP-mediated cell death involves CRT exposure, ATP and HMGB1 release, which are the principal damage-associated molecular patters (DAMPs) involved in ICD.