IFNG and cancer: In addition, a significant increase in IFN-γ release was observed in the co-culture of primed T cells with 4T1 cancer cells (192.29 ± 96.54 pg/mL), in comparison with the co-culture of unprimed T cells with 4T1 cells (8.44 ± 4.55 pg/mL) (Fig. 3g).