EFS and neoplasm: At each depth, expression levels of representative biomarkers of cellular apoptosis (caspase-3, termed as Cas-3), ICD (high mobility group box 1 protein, termed as HMGB1), and DC maturation (CD80 and CD86) were examined by immunofluorescent staining of tumor sections (Fig. 6d, Supplementary Figs. 26–28).