Moreover, signalling pathway enrichment analyses of the genes with differential expression revealed that tumour cytotoxic CD8+ T cell clusters (CD8_C7_GZMK, CD8_C8_MHC, and CD8_C9_XCL1) were enriched with the pathways related to cytokine production and lymphocyte activation; and CD8_C5_CX3CR1 was enriched with the pathways related to leukocyte trans-endothelial migration and leukocyte migration (Supplementary Fig. 4c), which are consistent with their capability in peripheral circulation and infiltrating to tumour24. Here, CD8A is linked to neoplasm.