These data sustain the concept that a single PNA is able to down-regulate not only the specific miRNA (in our case miR-335-5p), but also other miRNAs (for instance miR-155-5p), which might be involved in the regulation of the same pathway(s) (in our case, CFTR expression) or other pathways important in CF (i.e., the hyperinflammatory state characterized by overexpression of pro-inflammatory genes, including IL-8). Here, CFTR is linked to cystic fibrosis.