To gain insight into the cellular and molecular pathways primarily affected by the DM1 mutation in glial cells, in this study, we generated a DM1 model derived from human retinal Müller glial cells (MIO-M1) [31], which express a mutant DMPK RNA carrying 648 CUG repeats in an inducible manner [MIO-M1 CTG(648)]. The gene discussed is DMPK; the disease is myotonic dystrophy type 1.