Multicolor Flow Cytometry analysis of 9-marker multicolor panels in GBM patients (CD133, CD44, CD15, A2B5, CD36, CXCR4, IL6R, L1CAM, and ITGA6) revealed a phenotypic signature of CD44+/CD133+/ITGA6+/CD36+ of CSCs. This evidence concerns the gene CD44 and glioblastoma.