We used MV411, MOLM13, OCIAML3, and K562 AML cells that stably express Cas9 and transduced them with mCherry or GFP-expressing lentiviruses encoding specific and independent sgRNAs targeting CBP and EP300, as compared to the AAVS1 safe harbor locus and CDK1 that is required for cell survival, as negative and positive controls, respectively. The gene discussed is EP300; the disease is acute myeloid leukemia.