Gating on CD45RA−CD45RO+ circulating Tregs (as per our earlier strategy), we determined that PD-L1, IL-1R2, IL-21R, and CCR8 were generally absent (Supplementary Fig. 1a) and that the proportions of expressing cells were similar between CRC patients and healthy subjects (Supplementary Fig. 1b–e). This evidence concerns the gene IL1R2 and colorectal carcinoma.