CD274 and colorectal carcinoma: Gating on CD45RA−CD45RO+ circulating Tregs (as per our earlier strategy), we determined that PD-L1, IL-1R2, IL-21R, and CCR8 were generally absent (Supplementary Fig. 1a) and that the proportions of expressing cells were similar between CRC patients and healthy subjects (Supplementary Fig. 1b–e).