Using this same Treg gating strategy, we next examined the surface expression of eight protein markers (CD30, OX40, 4-1BB, TIGIT, PD-L1, IL-1R2, IL-21R, and CCR8) on tumor-infiltrating Tregs of five CRC patients from our South-East Asian cohort. This evidence concerns the gene TNFRSF4 and colorectal carcinoma.