ROC analyses of all Treg subsets revealed that CD45RO+CD30+OX40+, CD45RO+CD30−OX40+, and CD45RO+OX40+ Tregs could clearly discriminate between CRC patients and healthy subjects with an AUC > 0.9 and P < 0.0001 (Fig. 3f–h). The gene discussed is TNFRSF4; the disease is colorectal carcinoma.