Here, we report the kinetics of viral RNA shedding from different body sites and the concomitant antibody profile (IgM, IgA, IgG, and neutralizing Ig) along the disease course in the first 2 COVID-19 confirmed cases reported in Italy and hospitalized at the National Institute for Infectious Diseases “Lazzaro Spallanzani” (INMI) in Rome. Here, CD40LG is linked to COVID-19.