Next, we clustered FOXP3− CD4+ T cells derived of mice with and without TCL1 AT based on their expression of EOMES, PD-1, LAG3, and KI-67 measured by flow cytometry using T-distributed Stochastic Neighbor Embedding (t-SNE) algorithm to infer if CLL development is changing the phenotype of this cell type. The gene discussed is PDCD1; the disease is B-cell chronic lymphocytic leukemia.