Our observation is in agreement with a couple of reports, where the TRAIL-mediated apoptosis in human osteosarcoma cells41, nasopharyngeal carcinoma42, and mahanine-mediated apoptosis in human leukemic cells43 showed the reduction in the level of expression of Bid protein, which has been mentioned as the cleavage of Bid. This evidence concerns the gene BID and osteosarcoma.