Nevertheless, Michan et al. showed that neither overexpression of Sirt-1 by a genetic approach (i.e., conditional Sirt-1 overexpression in retinal neurons or vessels by breeding Sirt-1 overexpressing flox mice with Nestin-Cre or Tie2-Cre mice, respectively) or by a pharmacological approach with Sirt-1 agonists (i.e., RSV) provides additional protection against retinopathy in mice [168]. The gene discussed is SIRT1; the disease is retinal disorder.