To explore whether ROS regulate FOXC1-mediated HCC proliferation, migration and invasion, N-acetylcysteine (NAC) which is an antioxidant [33] and L-Buthionine-sulfoximine (BSO) which decreases GSH levels [34], were used to treat Huh7 -FOXC1 cells and MHCC97H-shFOXC1 cells, respectively. Here, FOXC1 is linked to hepatocellular carcinoma.