To explore whether DNMT3B was involved in FOXC1-mediated HCC proliferation and metastasis, we knocked down DNMT3B in Huh7-FOXC1 cells and ectopically upregulated DNMT3B expression in MHCC97H-shFOXC1 cells with lentivirus transfection (Fig. 2f). This evidence concerns the gene FOXC1 and hepatocellular carcinoma.