In this experiment, In addition, we found that the transcription factor SP1 can induce AGAP2-AS1,then we studied the expression of related target genes after knockdown of AGAP2-AS1 in CCA cells, and found that the expression of tumor suppressor gene CDKN1A was significantly increased after knockdown of AGAP2-AS1. This evidence concerns the gene AGAP2 and cholangiocarcinoma.