According to the survival analysis of the database, it was also shown that patients with high expression of AGAP2-AS1 had a shorter survival time (Fig. 1b) (https://starbase.sysu.edu.cn/).AGAP2-AS1) expression levels were determined in 32 pairs of paired CCA tumor tissues and adjacent tissues using qRT-PCR and normalized to GAPDH. This evidence concerns the gene GAPDH and cholangiocarcinoma.