The results showed that the allele frequencies of the PD-1 gene SNP loci (rs7421861 and rs10204525) and PD-L1 gene SNP loci (rs2282055, rs2890658, rs702275, and rs10125854) were significantly different between the two groups, indicating that these SNP loci may be related to the development of PCOS. Here, CD274 is linked to polycystic ovary syndrome.