In addition, in order to investigate whether the frequency distribution of genes and genotypes of PCOS patients and control groups has reached genetic balance and to understand whether they are representative of the population, we use the H-WE to detect the genotype frequency distribution of PD-1 gene SNP loci rs7421861 and rs10204525 and PD-L1 gene SNP loci rs2282055, rs2890658, rs702275, and rs10125854. This evidence concerns the gene CD274 and polycystic ovary syndrome.