Moreover, in a mice model with breast cancer, berberine loaded AgNPs were able to efficiently enter and disassemble the breast cancer cells cytoplasm to release its contents, i.e., nanoparticles and berberine, thereby, inducing cytotoxic effects and triggering apoptosis by hampering HIF-1α expression, through inhibition of PI3K/AKT and Ras/Raf/ERK proteins expression in signaling pathways, and generating ROS, respectively (Bhanumathi et al., 2018). This evidence concerns the gene AKT1 and breast carcinoma.