We further investigate the prognostic influence of tumor mutation burden (TMB, ≥2 mutations) and recurrent mutations, univariate analysis indicated that TMB ≥2, RUNX1 and JAK2 mutations were associated with inferior OS and PFS, and U2AF1 mutations was associated with inferior OS [hazard ratio (HR) > 1.5, p < 0.0500, Figure 2B]. The gene discussed is U2AF1; the disease is neoplasm.