To further analyze the effect of OTSSP167 on the proliferation of GBM cells in vivo and the expression levels of MELK and p-AKT(Ser473) in vivo, IHC was performed on GSC1 tumor tissues to detect the expression of MELK, p-AKT(Ser473) and Ki67. This evidence concerns the gene AKT1 and glioblastoma.