Although a synergistic effect of CLR with CFZ was demonstrated in a murine model of M. avium ATCC 700898 infection (Lanoix et al., 2020), our present study, to the best of our knowledge, is the first to evaluate CFZ activity against a chronic and progressive infection in a murine model that reflects the clinical situation, along with an investigation of its intracellular anti-MAC activity. Here, DCLK3 is linked to infection.