Concentrations of CFZ over 5 mg/L exhibited significant intracellular activity against both strains at 72 h post-infection in BMDMs despite the relatively high MIC values for M. avium ATCC 700898 and M. intracellulare ATCC 13950 compared to those for RIF and CLR (Figure 2A,B). This evidence concerns the gene DCLK3 and infection.