SIRT1 and Hyperglycemia: To further identify the molecular target of acacetin for the protection against high glucose- or hyperglycemia-induced vascular endothelial injury, siRNA molecules targeting Sirt1 or Sirt3 and the AMPK inhibitor dorsomorphin were utilized in HUVECs cultured with 33 mM glucose to determine their effects on acacetin-induced upregulation of Sirt1, PGC-1α, Sirt3, and pAMPK (Figures 6A,B).