To distinguish whether the synaptic Clu puncta are secreted from astrocytes or they are neuronal derived, we delivered low dose AAV-GFAP-Clu virus to the lateral ventricles of Clu knockout (KO) mouse brains at postnatal day 3 (P3) to sparsely label astrocytes [34], and examined the expression pattern of the astroglial expressed AAV-Clu in the absence of neuronal Clu 28 days post infection. The gene discussed is GFAP; the disease is infection.