TTN and hepatocellular carcinoma: To pinpoint the regulatory effects of TTN-AS1 on HCC cells’ multiplication, migration, invasion, and epithelial-mesenchymal transition (EMT), two shRNAs were used to knock down TTN-AS1 expression in HepG2 and Hep3B cells, respectively, and, as shown, both two shRNAs had high knockdown efficiency (Figure 2(a)).