To test how the three kinds of CAR T-specific CD30s mediated cell lysis, an LDH assay was used to measure the degree of injury to target cells (CD30-positive PTCL cell lines, Karpas 299 and SU-DHL-1; CD30-negative cell lines, Jurkat). The gene discussed is TNFRSF8; the disease is mature T-cell and NK-cell non-Hodgkin lymphoma.