It is also important to be mindful of potential stage-dependent invasion behaviors: in our previous study of early tumor spread of SD2 transplants, we found a significant vascular association of tumor cells at 14 dpi, which appeared to be reduced by Plexin-B2 knockdown22, while in this study with more advanced stages of GBM spread (i.e., ~150 to 200 dpi), we found that infiltrating GBM cells exhibited a predilection for axon tracts. This evidence concerns the gene PLXNB2 and glioblastoma.