Our finding that functional preservation by injections into the PSCC was only effective in early postnatal stages (P0–P2) is consistent with previous studies showing that only early postnatal interventions were able to yield positive results for hearing preservation in multiple mouse models of congenital hearing loss (e.g., Vglut3−/− mice9, Kcnq1−/− mice10, Ush1c c.216G>A mice24, and Otof−/− mice11). Here, USH1C is linked to hearing loss disorder.