Since TRAM2 expression showed significant high correlation with the YAP gene signature score in almost all tumor types (Fig. 2b), and TRAM2 is a component of the translocon at the endoplasmic reticulum (ER), we next decided to investigate changes in protein expression between MCF10A-TRAM2 (MCF10A cells ectopically expressing TRAM2), MCF10A-YAP5SA (MCF10A cells ectopically expressing activated YAP), and MF10A-Control (MCF10A cells ectopically expressing inactivated YAP,YAP5SA-S94A) cells using mass spectrometry (Fig. 4a, b; Additional file 6: Table S5). This evidence concerns the gene TRAM2 and neoplasm.