To assess the expression levels of B7-H3 in human primary tissues, we next performed IHC staining on the different types of Formalin-fixed paraffin-embedded (FFPE) human tumor (lung cancer, colon cancer, ﻿hepatocellular carcinoma, pancreatic cancer, kidney cancer, melanoma) and normal tissues (lung, colon, liver, pancreas, kidney) with the anti-B7-H3 antibody 8H9 and a goat antihuman B7-H3 antibody from R&D Systems. The gene discussed is CD276; the disease is melanoma.