In two xenografts involving A549 (high level of B7-H3) and NCI-H23 (low level of B7-H3), our results showed that BiKE was effective to suppress NSCLC growth in both models as was demonstrated with reduced tumor volume (75–80%), The in vivo activity of BiKE was not limited by B7-H3 antigen density of NCI-H23 at the low level. The gene discussed is CD276; the disease is neoplasm.