We first examined the expression of B7-H3 in a panel of tumor cell lines, including lung (A549, HCC827 and NCI-H23), breast (MDA-MA-231, BT-474 and HCC1954), colon (DLD-1 and HCT 116), ovarian (OVCAR-3 and SK-OV-3), acute myeloid leukemia (AML) (OCI-AML-3 and MOLM-13), pancreatic cancer (PANC-1), glioblastoma (SF188 and U251) and Burkitt's lymphoma (Daudi) cell lines, using flow cytometry and western blot assays. This evidence concerns the gene CD276 and familial pancreatic carcinoma.