As shown in Additional file 1: Fig. S1A, immunofluorescent staining showed that B7-H3 was strongly expressed in tumor cell lines (A549, HCC827, HCT-116, DLD1, MDA-MB-231, OCI-AML-3, BT-474, OVCAR-3, SK-OV-3, PANC-1, HCC1954, SF188 and U251), while NCI-H23 and MOLM-13 cells were weakly stained. This evidence concerns the gene CD276 and neoplasm.