They further established that cerebral ischemia facilitates hyper-phosphorylation of the WNK3-SPAK/OSR1 catalytic T-loop and of NKCC1 stimulatory sites (Thr203/Thr207/Thr212); thus, increased expression of NKCC1 in the brain cells [32]. The gene discussed is SLC12A2; the disease is Cerebral ischemia.