,28,36 Notably, NIS-fluorescent protein fusion reporters have been shown previously (1) to be correctly localized to the plasma membrane and function in several different cell types (rodent and human cancer cells,20,27, 28, 29,36 human CAR T cells,49 and hepatocyte-like cells29) and (2) to mediate uptake of various substrates similarly to unmodified NIS (for similar relative substrate uptake of NIS substrates, compare Figure S9A, Diocou et al.36 This evidence concerns the gene SLC5A5 and cancer.