CD4 and neoplasm: The cell densities and distance metrics within the tumor or parenchyma areas using tissues obtained from B95-8/fLuc-infected mice were analyzed (Figures 7A–C, representative example of immune cell phenotyping in a liver tumor and adjacent parenchyma by co-staining for DAPI, CD20, CD8, CD4, the proliferation marker Ki67, and the Treg marker FoxP3; for corresponding analyses in spleen tissue see Figure S3A).