To further substantiate the role of EVs-containing miR-25 in tumor growth and metastasis in vivo, SW620 cells were infected with lentivirus expressing OE-SIRT6 and treated with HCT116-EVs/HCT116-EVs-miR-25 agomir, while non-infected SW620 cells were treated with NCM460-EVs/HCT116-EVs/HCT116-EVs-miR-25 agomir. This evidence concerns the gene SIRT6 and neoplasm.