Furthermore, we treated ccRCC cell with Harmine, a naturally occurring beta-carboline alkaloid widely used herb, as a TWIST1 inhibitor selected through the unbiased screen and validated in the lung cells [33, 34], and found that it led to dramatic degradation of TWIST1 protein at either 10 μM or 40 μM while also drastically diminished VM formation in ccRCC cells. The gene discussed is TWIST1; the disease is nonpapillary renal cell carcinoma.