To examine the role of TWIST1 in ccRCC clinical samples, we detected its expression by IHC in 23 pairs of samples derived from ccRCC tumors and adjacent normal tissues (Fig. 2j) and the results showed a higher expression of TWIST1 was detected in tumors than in paired adjacent normal tissues (p < 0.001; n = 23) (Fig. 2k), which was consistent with the results extracted from TCGA mRNA database (Fig. 2l). The gene discussed is TWIST1; the disease is nonpapillary renal cell carcinoma.