Representative images of the histology and classification of tumour subtypes were included in Supplementary Figure 3B. Irrespectively to histology of the tumour of origin, iCCA cell cultures stably expressed mesenchymal and EMT markers (Fig. 1A), such as Vimentin, while on the contrary, cells expressed very low level (< 6% of cells) of the epithelial marker Cytokeratin-19 and were virtually negative for E-Cadherin (Fig. 1A) as demonstrated by immunofluorescence (IF). The gene discussed is KRT19; the disease is neoplasm.