To evaluate the impact of hnRNP A2B1 on tumorigenesis of melanoma stem cells in vivo, melanoma stem cells (A375 and MDA-MB-435) transfected with hnRNP A2B1-shRNA or hnRNP A2B1-shRNA-scrambled were injected into nude mice, followed by tumor examination (Fig. 5a). This evidence concerns the gene HNRNPA2B1 and melanoma.