To confirm the hnRNP-mediated splicing of TPPP3, EIF3H, DOCK2, DAPK1, RNF128, and SYT7 in melanoma stem cells, melanoma stem cells were transfected with hnRNP A2B1-siRNA or hnRNP A2B1-siRNA-scrambled, followed by the extraction of total RNAs and the detection of a randomly selected intron of a gene with semi-quantitative RT-PCR. The gene discussed is TPPP3; the disease is melanoma.